[ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

sigma. If you look at the Fo-Fc map, there are large areas of positive and negative density. The 2mFo-Fc map looks very convincing, you can see the tyrosines, phenyl rings, etc...Also phaser gave LLG of >400 and a TFZ of ~14. Rfree is ~0.45. Does anyone know what's going on? Thanks, Roger -- R

[ccp4bb] Coot Find Ligand Places Ligand In Density Outside Where Model Is Built

and not where the cursor was? I tried renaming the chains, but now it the find ligand doesn't even find anything even though there is clear density there. Thanks in advance. Roger -- Roger Shek Stony Brook University Graduate Student in Biochemistry and Structural Biology (PhD) Stony Brook Integrative