Re: [ccp4bb] off-topic: tag removal

[Srivastava, Dhiraj (MU-Student)]

See the following reference-

  Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, et al. 2009 
Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing 
Enzyme Tag. PLoS ONE 4(12): e8119. doi:10.1371/journal.pone.0008119



From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Philipp Ellinger 
[filu...@gmx.de]
Sent: Wednesday, February 23, 2011 3:17 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off-topic: tag removal

Dear all,

I have a question concerning removal of a his-tag sequence.
We have crystallized a protein with an important feature at the C-terminal part 
of the protein.
Unfortunately, we cannot express it with a N-terminal his-tag, only with a 
C-terminal his-tag.

Therefore we are looking for a protease which cleaves off the sequence without 
leaving any extra amino acid on the C-terminus of our protein. Meaning we 
obtain really the wild type protein.
Does anyone know about a protease or cleavage site which is completely removed?


Many thanks in advance

Phil

[ccp4bb] per-residue RMSD calculation for homologous structure

[Srivastava, Dhiraj (MU-Student)]

Hi All
   does anyone know any software that can calculate and print out RMSD of 
every residue (c alpha will be good) for homologous structures which has only 
30-40 % sequence similarity? I looked on the web but all the software that I 
found require the sequence to be the same for both structure.

Thank you

Dhiraj