[ccp4bb] Thanks

I have subscribed to the CCP4 bulletin board for over 25 years and attended many CCP4 meetings. I am changing role shortly and will no longer be a crystallographer so will unsubscribe from the board. I just wanted to pass on my thanks to everyone who has written posts, contributed to

[ccp4bb] Crystal Imager

Dear All, We are looking to replace our current Minstrel Rigaku Imager and would be interested to know what other people use and their unbiased opinion of the options available. Please feel free to contact me off the board and I will try and post a summary Thanks in advance Clare Dr Clare

Re: [ccp4bb] ITC question -dimer vs monomer

I would try it both ways and see what you get. Also do controls of buffer into each protein For extra info could also try with SPR. Always best to do these things using multiple complimentary methods Best wishes Clare From: CCP4 bulletin board On Behalf Of Bernhard Rupp Sent: 03 October

[ccp4bb] Vacancy for a Science Technician in Protein Crystallography and Biophysical Analysis at JIC, Norwich, UK

Dear All, I would like to draw your attention to an open position for a science technician supporting the protein crystallography and biophysical analysis facilities at the John Innes Centre in Norwich, UK. Details about the position (deadline for application: 16 Feb 2018) can be found here:

Re: [ccp4bb] advice on screen dispensing?

Olga, We use the liquidator96 and are very happy with it. You have to use their own tips but they fit well and as long as you use it properly it works really well. I trialled a few other makes but this works well for us. Best wishes Clare Dr Clare E.M. Stevenson John Innes Centre Norwich

Re: [ccp4bb] off topic: DLS instrument

Adam, We had a trial of the Avid nano W130i DLS system. I liked the small size and the disposable cuvettes. At present we have a Dynapro which is an old machine now but works well. Best wishes Clare From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tianyu Wang Sent: 17

Re: [ccp4bb] DNA Protein co- Crystallization

Dear Venkat, We recently solved a protein DNA complex structure and I had all the same queries as you have at the outset. We decided to use SPR as a techniques to study the interaction of the protein with the DNA and to use this to guide the choice of DNA for co-crystallisation. The work has

[ccp4bb] Summary - [ccp4bb] tips on crystallizing a Protein-DNA complex

Dear All, First I would like to thanks everyone who took the time to respond. It never fails to amaze me what an awesome forum the ccp4bb board is and how willing people are to share their expertise! Below is a summary of the replies I received 1. Do people routinely try different

[ccp4bb] tips on crystallizing a Protein-DNA complex

I was hoping people could give some tips on the best way to go about crystallizing a protein-DNA complex. I have a large amount of experience in protein crystallisation but have never tried co-crystallisation with DNA until I started this project. If you want to reply to me personally I will then