[ccp4bb] getting error while running Ample

[yogesh khandokar]

Dear ALL
Many thanks to my previous query. Can you please help me to fix error while
running Ample *(RuntimeError: Cannot find executable theseus in PATH.
Please give the path to theseus)*. The log file of error is attached
herewith.
Many thanks in advance
Regards

-- 
Yogesh Khandokar
PhD Student
School of Biomedical Sciences
Charles Sturt University
Wagga Wagga, NSW,
Australia
http://www.csu.edu.au/faculty/science/biomed/


28_ample.log
Description: Binary data

[ccp4bb] About protein stability

[yogesh khandokar]

Dear All,

I am working on enzyme which is expressed in Mycobacterium smegmatis. After
affinity chromatography and gel filtration, it is observed that enzyme stays
in dimeric form for 3hour approx. by the time it goes oligomeric form. i
want it to stay in dimeric form to get homogeneity of solution to set up
crystallization.
Required experts suggestions.

Very thankful to you in advance

*Elution Buffer of affinity chromatography*

50mm Phosphate pH7.4
200mm NaCl
1mM DTT
300 immidazole

*Gel filtration chromatography buffer*

20Mm phosphate pH7.4
100mM Nacl
2mM DTT

-- 
Yogesh Khandokar
SRF
Protein Crystallography Lab.,
National Institute of Immunology,
New Delhi.
India