[ccp4bb] On Behalf Of Katherine Sippel, Re: [ccp4bb] High Salt Cryo

2014-02-24 Thread Kiefersauer, Reiner
Hi, what was not mentioned in the ccp4 discussion is The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography Acta Cryst. (2012). D68, 69-81 I found it as a very potent compound for

Re: [ccp4bb] High Salt Cryo

2014-02-21 Thread Katherine Sippel
] From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine Sippel [katherine.sip...@gmail.com] Sent: Tuesday, February 18, 2014 12:08 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] High Salt Cryo Hi all, I'm looking for a cryo condition for high NaCl (3+ M

Re: [ccp4bb] High Salt Cryo

2014-02-21 Thread Keller, Jacob
if things don’t go the way you thought with MR. JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine Sippel Sent: Friday, February 21, 2014 11:48 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] High Salt Cryo I want to start off by thanking everyone

Re: [ccp4bb] High Salt Cryo

2014-02-19 Thread Mooers, Blaine H.M. (HSC)
Subject: [ccp4bb] High Salt Cryo Hi all, I'm looking for a cryo condition for high NaCl (3+ M) crystallization condition. I would do it the proper way, but our beam/cryostream is down. I've tried a bunch of things at the moment. Ethylene glycol and PEG 400 nuke the crystals immediately even at low

Re: [ccp4bb] High Salt Cryo

2014-02-19 Thread Karolina Michalska
] From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine Sippel [katherine.sip...@gmail.com] Sent: Tuesday, February 18, 2014 12:08 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] High Salt Cryo Hi all, I'm looking for a cryo condition for high

Re: [ccp4bb] High Salt Cryo

2014-02-19 Thread Enrico Stura
bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine Sippel [katherine.sip...@gmail.com] Sent: Tuesday, February 18, 2014 12:08 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] High Salt Cryo Hi all, I'm looking for a cryo condition for high NaCl (3+ M) crystallization condition. I

[ccp4bb] High Salt Cryo

2014-02-18 Thread Katherine Sippel
Hi all, I'm looking for a cryo condition for high NaCl (3+ M) crystallization condition. I would do it the proper way, but our beam/cryostream is down. I've tried a bunch of things at the moment. Ethylene glycol and PEG 400 nuke the crystals immediately even at low concentrations. Prolonged

Re: [ccp4bb] High Salt Cryo

2014-02-18 Thread Roger Rowlett
How about a short swish in well solution + 25-30% glucose? Doesn't take long to cryoprotect, just a quick sufrace coat. Sodium malonate? We just froze some really fragile crystals from 1.8 M sodium formate in 3 M sodium malonate and they held up really well. (Still didn't improve their