Hi,
what was not mentioned in the ccp4 discussion is
The use of trimethylamine N-oxide as a primary
precipitating agent and related methylamine
osmolytes as cryoprotective agents for
macromolecular crystallography
Acta Cryst. (2012). D68, 69-81
I found it as a very potent compound for
]
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Katherine Sippel [katherine.sip...@gmail.com]
Sent: Tuesday, February 18, 2014 12:08 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] High Salt Cryo
Hi all,
I'm looking for a cryo condition for high NaCl (3+ M
if things
don’t go the way you thought with MR.
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine
Sippel
Sent: Friday, February 21, 2014 11:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] High Salt Cryo
I want to start off by thanking everyone
Subject: [ccp4bb] High Salt Cryo
Hi all,
I'm looking for a cryo condition for high NaCl (3+ M) crystallization
condition. I would do it the proper way, but our beam/cryostream is down.
I've tried a bunch of things at the moment. Ethylene glycol and PEG 400 nuke
the crystals immediately even at low
]
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Katherine
Sippel [katherine.sip...@gmail.com]
Sent: Tuesday, February 18, 2014 12:08 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] High Salt Cryo
Hi all,
I'm looking for a cryo condition for high
bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Katherine Sippel [katherine.sip...@gmail.com]
Sent: Tuesday, February 18, 2014 12:08 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] High Salt Cryo
Hi all,
I'm looking for a cryo condition for high NaCl (3+ M) crystallization
condition. I
Hi all,
I'm looking for a cryo condition for high NaCl (3+ M) crystallization
condition. I would do it the proper way, but our beam/cryostream is down.
I've tried a bunch of things at the moment. Ethylene glycol and PEG 400
nuke the crystals immediately even at low concentrations. Prolonged
How about a short swish in well solution + 25-30% glucose? Doesn't take
long to cryoprotect, just a quick sufrace coat. Sodium malonate? We just
froze some really fragile crystals from 1.8 M sodium formate in 3 M
sodium malonate and they held up really well. (Still didn't improve
their