Re: [ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure

2020-06-22 Thread Alan Cheung
Thanks everyone for recommendations. The AKTA currently lives in a cold room hence all of our purifications have been optimised at 4C, and i'm reluctant to transition them to RT. Also, most of our columns are not jacketed. On Sun, 21 Jun 2020 at 18:19, Boniecki, Michal wrote: > Hi Alan,

Re: [ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure

2020-06-21 Thread Boniecki, Michal
Hi Alan, I think new AKTA collector can be refrigerated. If this is not an option, consider putting only collector in the cold cabinet and cooling down the buffers. Also, you can put only columns and fraction collector inside cabinet which has side port for such use. Although you will have to

[ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure

2020-06-21 Thread Hughes, Jonathan
hi alan, why do you want to put the thing in a cold cabinet? if you have protease problems, there are better ways to get rid of them than exploiting Q10. on the other hand, you'll increase viscosity and thus slow down the flow rates. also, the high humidity will reduce the lifetime of your Äkta