Re: [ccp4bb] Antw: Re: [ccp4bb] Ramachandran oultliers increase upon restrained refinement

Of course - none of us has given the best advice of all! Try to find a better ordered crystal! Eleanor On 2 July 2017 at 19:59, Matthias Barone wrote: > Beside the good propositions of fixing the environment, flip the peptide, > or using PDB_REDO, I would suggest the

[ccp4bb] Antw: Re: [ccp4bb] Ramachandran oultliers increase upon restrained refinement

Beside the good propositions of fixing the environment, flip the peptide, or using PDB_REDO, I would suggest the following additional attempts: Try 1) setting the OCC of the sidechain (CG and further) below 0.3 or mutate directly to Ala. See which ones remain visible in a diff map and bring