: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of F.
Timur Senguen
Sent: 18 July 2011 14:53
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Bypassing phase separation for nice crystals.
Hi everyone,
I have been issues with a particular protein. I have been close for a
while, but yet so
Thanks for all the suggestions.
I forgot to mention that I had tried microseeding and it was partially
successful. I am trying this while also pursuing other venues. At Florian's
suggestion I have set up a free interface diffusion method and it looks very
promising. I have a few free floating
Hi everyone,
I have been issues with a particular protein. I have been close for a while,
but yet so far.
Rather than going from a clear drop to crystal, my protein first undergoes
phase separation (large oily drops) in which one phase contains most, if not
all, of the protein. This phase
Hi Timur,
have you tried seeding from your microstalline stuff? Might be worth to try!
Cheers,
Albert
Albert GUSKOV (Dr) | Research Fellow | Division of Structural
Computational Biology | Nanyang Technological University
Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 GMT+8h
Original-Nachricht
Betreff:Re: [ccp4bb] Bypassing phase separation for nice crystals.
Datum: Mon, 18 Jul 2011 17:01:27 +0200
Von:Florian Sauer sa...@embl-hamburg.de
An: F. Timur Senguen ftseng...@gmail.com
CC: CCP4BB@JISCMAIL.AC.UK
Dear Timur,
one
