Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

2017-01-27 Thread pselvam
Dear All, thanks for your replies. I tend to believe that it could be ATP some how copurified with the protein. Refinement with ATP, ADP+acetate, did not vary too much in the B factors. The main concern is, only one xtal was observed at this state ( mimicked like a ATP bound state and

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

2017-01-19 Thread Phoebe A. Rice
To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) Remember that 2xADP can disproportionate into ATP + AMP > On 19 Jan 2017, at 20:54, Panneerselvam, Saravanan > <saravanan.panneersel...@desy.de> wrote: > > Dear All, > Sor

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

2017-01-19 Thread Phil Evans
..@jiscmail.ac.uk> > To: CCP4BB@JISCMAIL.AC.UK > Sent: Sunday, 15 January, 2017 01:01:37 > Subject: CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) > > There are 2 messages totaling 45 lines in this issue. > > Topics of the day: > > 1. Phenix (2) > > -

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

2017-01-19 Thread Phil Jeffrey
On 1/19/17 3:54 PM, Panneerselvam, Saravanan wrote: We observed additional density around ADP that fits perfectly like a gamma phosphate Hello Saravanan At 1.4 Angstrom resolution wouldn't that suggest that you've somehow got ATP in there ? I don't think I understand the other option - were

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

2017-01-19 Thread Panneerselvam, Saravanan
ystem" <lists...@jiscmail.ac.uk> To: CCP4BB@JISCMAIL.AC.UK Sent: Sunday, 15 January, 2017 01:01:37 Subject: CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) There are 2 messages totaling 45 lines in this issue. Topics of the day: