Fatty acids are very soluble in glycerol or the PEGs.
Most proteins tolerate high concentrations (30-50%) glycerol and PEGs.�
So, it is quite possible to make a solution that will allow the protein
crsytal and the ligand to both be in the same solution.
One of my former colleagues was in
Hi Frank,
heme becomes near completely insoluble when it dimerizes. Fatty acids
are also very insoluble. It is quite hard to get them into HSA, as far
as I remember.
Cheers,
Tim
On Wed, 23 Jun 2021 10:33:42 +0100 Frank von
Delft wrote:
> And then of course, you need to decide whether you at
And then of course, you need to decide whether you at all care to know
anything about a compound that is so insoluble that it needs that kind
of treatment :)
On 23/06/2021 09:52, hoh wrote:
Hi
As total insolubility does not exist, I regularly use another method,
which is to deposit a
Hi
As total insolubility does not exist, I regularly use another method,
which is to deposit a grain of the ligand directly into the drop.
In this case, we no longer control the concentration. The goal is to
regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the
results, it
udhury
mailto:goura...@csiriicb.res.in>>
*Sent:* Saturday, April 24, 2021 12:46 PM
*To:* CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
*Subject:* [ccp4bb] Co crystalization with less soluble ligand.
Hello everyone,
I
of Gourab Basu
Choudhury
Sent: 24 April 2021 03:46
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Co crystalization with less soluble ligand.
External Sender: Use caution.
Hello everyone,
I am finding it difficult for getting a ligand density inside the protein as
the ligand is very much insoluble
Dear Gourab,
You might just want to add the ligand in pure, solid form to the edge of the
crystallization drop. This worked in our case beautifully (see Angew. Chem.
Int. Ed. 2012, 51, 3354). We had to soak it for 4 weeks.
Good luck!
Best regards
Bernhard
L.AC.UK
Emne: Re: [ccp4bb] Co crystalization with less soluble ligand.
I tried sokaing ,high concentration of DMSO is effecting crystal,
so I tried other contidtion where 10%DMSO is there. Got 1.9 A
data with protein co crystalization with 10mM ligand. But ligand
occupancy is not visble.
I got the K
g/index.php/User:Casper_Wilkens
>>> https://twitter.com/protein_artist
>>> https://www.researchgate.net/profile/Casper_Wilkens
>>> https://www.cazypedia.org/index.php/User:Casper_Wilkens
>>>
>>>
---------------
>>
>> Fra: CCP4 bulletin board på vegne af Gourab Basu
>> Choudhury
>> Sendt: 24. april 2021 07:40:20
>> Til: CCP4BB@JISCMAIL.AC.UK
>> Emne: Re: [ccp4bb] Co crystalization with less soluble ligand.
>
rade+%0D%0AParkville,+VIC,+3052?entry=gmail=g>
>> Parkville, VIC, 3052
>> <https://www.google.com/maps/search/343+Royal+Parade+%0D%0AParkville,+VIC,+3052?entry=gmail=g>
>> +613 9662 7304
>> +614 57 539 419
>> tom.p...@csiro.au
>>
>> -
BB@JISCMAIL.AC.UK
Emne: Re: [ccp4bb] Co crystalization with less soluble ligand.
I tried sokaing ,high concentration of DMSO is effecting crystal, so I tried
other contidtion where 10%DMSO is there. Got 1.9 A data with protein co
crystalization with 10mM ligand. But ligand occupancy is not visbl
RO
> 343 Royal Parade
> Parkville, VIC, 3052
> +613 9662 7304
> +614 57 539 419
> tom.p...@csiro.au
>
> --
> *From:* CCP4 bulletin board on behalf of Gourab
> Basu Choudhury
> *Sent:* Saturday, April 24, 2021 12:46 PM
> *To:* CCP4BB@JISCMAI
@JISCMAIL.AC.UK
Subject: [ccp4bb] Co crystalization with less soluble ligand.
Hello everyone,
I am finding it difficult for getting a ligand density inside the protein as
the ligand is very much insoluble. It's only soluble in 100% DMSO. I tried for
co crystalization. Kd value of the ligand
Hello everyone,
I am finding it difficult for getting a ligand density inside the protein
as the ligand is very much insoluble. It's only soluble in 100% DMSO. I
tried for co crystalization. Kd value of the ligand is near 40um. Any
suggestion what to do?
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