PROTECTED]>
>Subject: Re: [ccp4bb] Cryoprotectant for protein-DNA complex
crystal
>To: CCP4BB@JISCMAIL.AC.UK
>
>Why soak for a whole minute? A single pass through cryo is
usually enough,
>and that takes a couple of seconds with the right set-up...
>
>You could try oil - if you
PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Cryoprotectant for protein-DNA complex crystal
Why soak for a whole minute? A single pass through cryo is usually enough, and
that takes a couple of seconds with the right set-up...
You could try oil - if you're lucky it solves your issues.
Try soaking for about 5 seconds. You should only need to coat the
surface of the crystal to cryoprotect it.
Bill
On Oct 31, 2008, at 2:22 PM, E rajakumar wrote:
When I soak the
crystals more than a minute
bject: [ccp4bb] Cryoprotectant for protein-DNA complex crystal
Dear All
I am working on protein-DNA complex crystals for data
collection. These crystals are grown in 15-20 % of
PEG3350 or PEG4000 with pH of 6 to 7. When I soak the
crystals more than a minute in the cryo solution
(15-20% of Glycer
Dear All
I am working on protein-DNA complex crystals for data
collection. These crystals are grown in 15-20 % of
PEG3350 or PEG4000 with pH of 6 to 7. When I soak the
crystals more than a minute in the cryo solution
(15-20% of Glycerol or ethylenglycol + reservoir) the
resolution of diffraction is
