If your precipitant is high concentration salt, there will be a significant
freezing point depression (see e.g. wikipedia entry
http://en.wikipedia.org/wiki/Freezing-point_depression ). I seem to
remember collecting data from crystals we cooled to -15 C in the early
'80s. The crystals were grown i
Hi Glenn -
I have nothing systematic, but I remember that transducin-alpha (1TND)
was crystallized at -12 C, with 20% glycerol in the mother liquor. (The
crystals grew at higher temperatures, but weren't as good.) I worked on
this project briefly in grad school, and I remember that looking a
: Thursday, May 30, 2013 6:26 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystallisation below 0°C
Hello CCP4BBers,
I am currently playing with some crystals that seem to enjoy lower
temperatures, and I was thinking of breaking the 0°C threshold.
...
Glenn Masson,
We had crystals that appeared by chance at "underfined" low temparature:
Maïga, A., Vera, L., Marchetti, C., Lorphelin, A., Bellanger, L., Mourier,
G., Servent, D., Gilles, N. & Stura, E. A. (2013) Crystallization of
recombinant Green mamba ρ-Da1a toxin during lyophilization pro
Hello CCP4BBers,
I am currently playing with some crystals that seem to enjoy lower
temperatures, and I was thinking of breaking the 0°C threshold.
Looking for examples of this in the literature is problematic, as searching
for examples in the PDB (Under the advanced search-> crystal properties->
