Sabine,
There are protocols to modify surface residues that can help with
crystallization, and make the protein less soluble. Unfortunately, I¹m
drawing a blank on the details. I remember someone in Andrzej Joachimiak¹s
group was working on this as a rescue approach for the structural genomics
Hello, Sabine.
I believe the technique that Kendall is referring to is Surface Entropy
Reduction, which involves replacing large, highly entropic residues on the
surface of the protein with lower entropy residues to reduce the entropy
shield and facilitate crystallization. Traditionally, the
Hello Sabine,
Maybe this is an option:
Structure, Vol 14, 1617-1622, November 2006
Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization
best regards good luck
Christian
Schneider Sabine wrote:
Hi everyone,
I am trying to crystallise an extremely soluble and
