Re: [ccp4bb] DNA oligonucleotide purification

2011-04-28 Thread Phoebe Rice
Original message >Date: Thu, 28 Apr 2011 08:32:23 -0500 >From: CCP4 bulletin board (on behalf of "Wallen, >Jamie" ) >Subject: [ccp4bb] DNA oligonucleotide purification >To: CCP4BB@JISCMAIL.AC.UK > > All, > > Our laboratory is looking for a n

Re: [ccp4bb] DNA oligonucleotide purification

2011-04-28 Thread Kushol Gupta
We're using an older Dynamax system that is outfitted with PEEK throughout. Cheers, Kushol From: Wallen, Jamie [mailto:jwal...@biochem.wustl.edu] Sent: Thursday, April 28, 2011 10:17 AM To: Kushol Gupta; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] DNA oligonucleotide purific

Re: [ccp4bb] DNA oligonucleotide purification

2011-04-28 Thread Wallen, Jamie
Title: Re: [ccp4bb] DNA oligonucleotide purification Kushol, Thank you for the reply. Might I ask what kind of HPLC system you have? Our HPLC is old and has stainless steel pumps, and to our knowledge this is not compatible with the Dionex columns (due to metal leaching). Dionex has

Re: [ccp4bb] DNA oligonucleotide purification

2011-04-28 Thread Kushol Gupta
penn.edu 215-573-7260 / 267-259-0082 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Wallen, Jamie Sent: Thursday, April 28, 2011 9:32 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] DNA oligonucleotide purification All, Our laboratory is looking for a new column t

[ccp4bb] DNA oligonucleotide purification

2011-04-28 Thread Wallen, Jamie
Title: DNA oligonucleotide purification All, Our laboratory is looking for a new column to purify short (40mers or less) DNA oligonucleotides that will be used for crystallization setups. I wanted to ask if anyone has suggestions of a column that will give excellent separation of N and N-1 prod