Hi,
I would like ask your advice on designing SUMO fusion proteins.
Specifically, is it recommended to include a linker between the
C-terminus of SUMO (-QIGG) and the N-terminus of the target protein?
Figure 3 of Guerrero et al. (https://pubmed.ncbi.nlm.nih.gov/26297996/
Hi Jack,
We routinely include a single glycine downstream of the SUMO (so, …QIGGG). This
essentially always gives good cleavage with UD, assuming the construct isn’t
aggregated or otherwise unhappy.
Re the His tag: Presumably you mean at the N-terminus of SUMO? In this case
we’ve had good
Sorry for the non-CCP4 question…
I would like ask your advice on designing SUMO fusion proteins. Specifically,
is it recommended to include a linker between the C-terminus of SUMO (-QIGG)
and the N-terminus of the target protein? Figure 3 of Guerrero et al.
