[ccp4bb] Differentiate salt and protein crystals

2012-03-15 Thread Theresa H. Hsu
Hi all. I set up some trays of membrane protein remotely in cubic phase. I don't have ready access to them so I can't shoot/pick the crystals. Under polarising lights, some crystals appears coloured across many conditions, making me think these are salt. Is there some knowledge of inorganic

Re: [ccp4bb] Differentiate salt and protein crystals

2012-03-15 Thread Jingquan Tan
Hi Theresa, Your observation of colored crystals under polarising lights seems odd to me. I assume your protein should be colorless and under normal light these crystals are colorless? Are these trays set up in glass plate or plastic plates? If you are willing to upload some pictures, I might be

Re: [ccp4bb] Differentiate salt and protein crystals

2012-03-15 Thread Jon Agirre
The best reproduction I can suggest would be to setup one or two LCP experiments exchanging the protein for its buffer. If you get crystals, you know for sure they're not protein. Cheers, Jon 2012/3/15 Theresa H. Hsu theresah...@live.com Hi all. I set up some trays of membrane protein

Re: [ccp4bb] Differentiate salt and protein crystals

2012-03-15 Thread Jacob Keller
On Thu, Mar 15, 2012 at 11:14 AM, Jon Agirre jon.agi...@gmail.com wrote: The best reproduction I can suggest would be to setup one or two LCP experiments exchanging the protein for its buffer. If you get crystals, you know for sure they're not protein. It should be pointed out that the