Hi Appu,
Adding the extinction coefficients of the nucleotides is a reasonable
approximation; a somewhat more rigorous approximation takes into account
nearest neighbor frequencies; see, for example, some commercial web sites such
as
Hello Appu,
Small amounts of strongly UV absorbing contaminants can throw off
your measurements. If you want to be very accurate, try a fluorescent
dye specific for double stranded DNA such as PicoGreen.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of
Deal CCP4 member's
Sorry for asking Off topic question.
Please correct me if i am doing wrong measurements. I am determining the
double stranded concentration using the absorbance spectrophotometer
reading at 260A and extinction coefficients for individual
