Re: [ccp4bb] Formulation of my own mother liquor
Well I think some companies make the screens from stocks, the others adjust the pH of the final mixture, so you might want to check the small print for the screen you used in the first place… But when optimizing with your own solutions, you would in general take the easy way out - make good stocks and mix them as you wish. Petri Petri Kursula, PhD -- Professor of Biochemistry and Molecular Biology Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/persons/Petri.Kursula http://www.uib.no/en/persons/Petri.Kursula petri.kurs...@biomed.uib.no mailto:petri.kurs...@biomed.uib.no -- Project Leader, Docent Faculty of Biochemistry and Molecular Medicine Biocenter Oulu University of Oulu, Finland petri.kurs...@oulu.fi mailto:petri.kurs...@oulu.fi -- On 21 Nov 2014, at 17:48, amro selem 03391c09f749-dmarc-requ...@jiscmail.ac.uk wrote: Dear All, first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT) the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff together and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8. any suggest any idea or any one knows what the company do. CHEERS Amr
[ccp4bb] Formulation of my own mother liquor
Dear All,first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT)the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff together and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8.any suggest any idea or any one knows what the company do. CHEERSAmr
Re: [ccp4bb] Formulation of my own mother liquor
Generally one will make this up from stock solutions: Make up 1 M Tris chloride pH 8 1 M magnesium chloride 50 % (weight/volume) PEG 6K Then mix these together in the right ratios (for 1 mL) 100 uL 1 M tris solution 200 uL 1 M MgCl2 solution 400 uL 50% PEG solution 300 uL water. Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email janet.new...@csiro.au From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of amro selem [03391c09f749-dmarc-requ...@jiscmail.ac.uk] Sent: 22 November 2014 03:48 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Formulation of my own mother liquor Dear All, first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT) the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff together and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8. any suggest any idea or any one knows what the company do. CHEERS Amr
Re: [ccp4bb] Formulation of my own mother liquor
The most important thing is that you do it the same way every time. But you might also put some thought into flexibility, in case you need to explore cryoprotectants, etc. On 11/21/14 11:48, amro selem wrote: Dear All, first i wish you nice weekend, then i wanna ask about formulation of my own mother liquor, i want to optimize a condition containing 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT) the question is , should i make a stock solution from every ingredient by dissolving it in miliQ water. then mix all stuff together and add water till final concentration with out adjusting final pH. OR dissolve the PEG in pH adjusted buffer and then mix all ingredients , so the final pH will be 8. any suggest any idea or any one knows what the company do. CHEERS Amr -- === All Things Serve the Beam === David J. Schuller modern man in a post-modern world MacCHESS, Cornell University schul...@cornell.edu
