Dear All,
Greetings and apology for an off topic query. may request you all to kindly
share with me a conventional protocol, by which i can calculate a total
Glutathione content (oxidized + reduced) in an *in-vitro* reaction set up.
all i find over internet are kit-based methods, which turns out
Dear Avinash,
you can use Ellman's reagent (DTNB) for GSH.
Have a look here:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
For glutathione you don't have to use a buffer with GuHCl.
HTH, Guenter
Dear All,
Greetings and apology for an off topic query. may
