[ccp4bb] How to calculate data collection strategy manually?
How to calculate data collection strategy manually. I was wondering if any one can answer this, if we have to calculate data collection strategy manually? regards Saleem
Re: [ccp4bb] How to calculate data collection strategy manually?
Dear Saleem, To compute one manually I take it you mean by hand with commonly available software as opposed to via e.g. automated beamline methods? If so: start up imosflm index your image(s) and estimate mosaic spread select strategy, auto-complete, go XDS has XPLAN equivalently, though you need to be careful to set the symmetry. Best wishes, Graeme On 26 March 2013 12:25, saleem raza mysaleemr...@hotmail.com wrote: How to calculate data collection strategy manually. I was wondering if any one can answer this, if we have to calculate data collection strategy manually? regards Saleem
Re: [ccp4bb] How to calculate data collection strategy manually?
Hi Saleem I can strongly recommend reading and digesting this paper (open access from the IUCr website, so you don't need a subscription) - Dauter, Z., Acta Cryst. (1999). D55, 1703-1717 - Data-collection strategies http://journals.iucr.org/d/issues/1999/10/00/ba0020/ba0020.pdf Once you've read it, the issues that you should take into account when devising your strategy shuld be apparent. Of course, all the best integration programs also have strategy options built into them as well if you decide not to do it manually after all On 26 Mar 2013, at 12:25, saleem raza wrote: How to calculate data collection strategy manually. I was wondering if any one can answer this, if we have to calculate data collection strategy manually? regards Saleem Harry -- ** note change of address ** Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] How to calculate data collection strategy manually?
Yes, I highly highly recommend the Dauter (1999) paper that Harry has suggested. Also, check out: Dauter Z. Efficient use of synchrotron radiation for macromolecular diffraction data collection. Prog Biophys Mol Biol. 2005 Oct;89(2):153-72. Most everything you want to learn about data collection is in these two papers. Cheers, Raji On Tue, Mar 26, 2013 at 9:06 AM, Harry Powell ha...@mrc-lmb.cam.ac.ukwrote: Hi Saleem I can strongly recommend reading and digesting this paper (open access from the IUCr website, so you don't need a subscription) - Dauter, Z., Acta Cryst. (1999). D55, 1703-1717 - Data-collection strategies http://journals.iucr.org/d/issues/1999/10/00/ba0020/ba0020.pdf Once you've read it, the issues that you should take into account when devising your strategy shuld be apparent. Of course, all the best integration programs also have strategy options built into them as well if you decide not to do it manually after all On 26 Mar 2013, at 12:25, saleem raza wrote: How to calculate data collection strategy manually. I was wondering if any one can answer this, if we have to calculate data collection strategy manually? regards Saleem Harry -- ** note change of address ** Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
[ccp4bb] Fwd: [Err] Re: [ccp4bb] How to calculate data collection strategy manually?
Not sure why I keep getting this message from an email id in korea (see below). Are other members getting this email as well? Is someone someone at ccp4bb able to stop this id from spamming me everytime I send emails to the BB. Thanks. Raji -- Forwarded message -- From: spam_mas...@korea.ac.kr Date: 2013/3/26 Subject: [Err] Re: [ccp4bb] How to calculate data collection strategy manually? To: r...@brandeis.edu Transmit Report: yangr...@korea.ac.kr에게 메일 발송을 5번 시도했지만 실패하였습니다. (실패 이유 : 554 Transaction failed. 402 Local User Inbox Full ( yangr...@korea.ac.kr) 8,61440,240522(163.152.6.98)) 참고 실패 이유에 대한 설명 User unknown :메일을 수신할 사용자가 존재하지 않음 Socket connect fail:수신 메일 서버와 연결 실패 DATA write fail:수신 메일 서버로 메세지 송신 실패 DATA reponse fail :수신 메일 서버로부터 메세지 수신 실패 Final-Recipient: rfc822;yangr...@korea.ac.kr Diagnostic-Code: smtp; 554 error - Transaction failed. 402 Local User Inbox Full (yangr...@korea.ac.kr) 8,61440,240522(163.152.6.98) Action: failed Status: 4.0.0 -- Forwarded message -- From: Raji Edayathumangalam r...@brandeis.edu To: CCP4BB@JISCMAIL.AC.UK Cc: Date: Tue, 26 Mar 2013 10:17:35 -0400 Subject: Re: [ccp4bb] How to calculate data collection strategy manually? Yes, I highly highly recommend the Dauter (1999) paper that Harry has suggested. Also, check out: Dauter Z. Efficient use of synchrotron radiation for macromolecular diffraction data collection. Prog Biophys Mol Biol. 2005 Oct;89(2):153-72. Most everything you want to learn about data collection is in these two papers. Cheers, Raji On Tue, Mar 26, 2013 at 9:06 AM, Harry Powell ha...@mrc-lmb.cam.ac.ukwrote: Hi Saleem I can strongly recommend reading and digesting this paper (open access from the IUCr website, so you don't need a subscription) - Dauter, Z., Acta Cryst. (1999). D55, 1703-1717 - Data-collection strategies http://journals.iucr.org/d/issues/1999/10/00/ba0020/ba0020.pdf Once you've read it, the issues that you should take into account when devising your strategy shuld be apparent. Of course, all the best integration programs also have strategy options built into them as well if you decide not to do it manually after all On 26 Mar 2013, at 12:25, saleem raza wrote: How to calculate data collection strategy manually. I was wondering if any one can answer this, if we have to calculate data collection strategy manually? regards Saleem Harry -- ** note change of address ** Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
