Re: [ccp4bb] How to reduce R-factor and Free-R

2009-12-18 Thread Francis E Reyes 
What is the space group? Is there unmodeled density? Are all residues  
in your construct built? What is the refinement protocol?


FR

On Dec 18, 2009, at 6:47 AM, james09 pruza   
wrote:



Dear All,

I am trying to solve a 2.55 A resolution data set. The R-factor is  
around 24% while Free-R is 30%. The Residues in most favourable  
region is 95% while additionally allowed region is 5%. What are the  
ways to reduce the R-factor and Free-R? Around 100 water molecules  
are placed.


Thanks in advance for the help.

J...

[ccp4bb] How to reduce R-factor and Free-R

2009-12-18 Thread james09 pruza 
Dear All,

I am trying to solve a 2.55 A resolution data set. The R-factor is around
24% while Free-R is 30%. The Residues in most favourable region is 95% while
additionally allowed region is 5%. What are the ways to reduce the R-factor
and Free-R? Around 100 water molecules are placed.

Thanks in advance for the help.

J...