Dear All,
Thanks for all the replies on and off-board. I received around twenty replies
and the majority have spoken in favor of the QIAgen BSA-free anti-5His mAb from
QIAGEN. Not to be bias, a couple of people recommended the one from Abcam as
well.
Thanks again, Dan
[CCP4BB@JISCMAIL.AC.UK] on behalf of D Bonsor [
dbon...@ihv.umaryland.edu]
Sent: Monday, June 25, 2012 23:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never
used one and I heard several stories
[dbon...@ihv.umaryland.edu]
Sent: Monday, June 25, 2012 23:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never used
one and I heard several stories that they were poor and not worth the money,
over
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never
used one and I heard several stories that they were poor and not worth the
money, over the past few years. The only post I could find on the BB was
from 2008
Are there any good antibodies for His-tags on the market. I have never used one
and I heard several stories that they were poor and not worth the money, over
the past few years. The only post I could find on the BB was from 2008. Have
His-Antibodies improved or are they still rubbish.
The one we have is rubbish. I can tell a vendor name if you want off-list.
A lot of non-specific bands + high background level makes it almost
impossible to use against cell lysate. It sort of works against purified
His-tag protein, but not perfect either.
On Mon, Jun 25, 2012 at 4:33 PM, D
@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never used one
and I heard several stories that they were poor and not worth the money, over
the past few years. The only post I could find on the BB was from 2008. Have
His-Antibodies
] on behalf of D Bonsor
[dbon...@ihv.umaryland.edu]
Sent: Monday, June 25, 2012 23:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never used one
and I heard several stories that they were poor and not worth the money
