Re: [ccp4bb] On maps and doubts

2012-10-12 Thread Jan Dohnalek
might want to try the Buster > program. > > Best regards, > Herman > > -- > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Israel > Sanchez > *Sent:* Thursday, October 04, 2012 9:17 PM > *To:* CCP4BB@JISCM

Re: [ccp4bb] On maps and doubts

2012-10-10 Thread Jacob Keller
btw, this thread has one of my favorite titles ever... JPK On Tue, Oct 9, 2012 at 4:11 AM, Eleanor Dodson wrote: > This is interesting. In principle m and D should provide an optimum map, > and at high resolution they do a reasonable job. > The answer about occupancy is a good point. > > You do

Re: [ccp4bb] On maps and doubts

2012-10-09 Thread Eleanor Dodson
This is interesting. In principle m and D should provide an optimum map, and at high resolution they do a reasonable job. The answer about occupancy is a good point. You don't say what resolution your data is at, but maybe it is rather low? I suspect that below ~ 3A the estimates of both m and

Re: [ccp4bb] On maps and doubts

2012-10-04 Thread Herman . Schreuder
program. Best regards, Herman From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Israel Sanchez Sent: Thursday, October 04, 2012 9:17 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] On maps and doubts

Re: [ccp4bb] On maps and doubts

2012-10-04 Thread Bosch, Juergen
Try GraphEnt but you might have to recompile it for your size of proteins. That has helped me several times to see something that was barely there. Here's the link for the 'click-generation': http://utopia.duth.gr/~glykos/graphent.html Jürgen .. Jürgen Bosch Johns Hopkins Un

[ccp4bb] On maps and doubts

2012-10-04 Thread Israel Sanchez
Hello everyone, I would like to share my experience with one dataset and request some advice on which is the best way to prove a conformational change seen in a density map. The first issue arose when we were looking for an extra ribosomal factor added to a crystalized ribosome. After careful dat