Dear all
I am interested in crystallizing a 5 residue peptide. I have no prior
experience in this field although I have crystallized larger proteins (5--60
kDa). Do people use regular screens used in macromolecular crystallization
such as Hamptons, wizards etc? Any suggestions are greatly
On Tue, 2011-09-06 at 11:51 -0500, Chaudhary, Ritcha wrote:
Do people use regular screens used in macromolecular crystallization
such as Hamptons, wizards etc?
Probably not - I believe short peptides are crystallized mostly via
various evaporation techniques (but I am very curious to hear what
Dear Ritcha,
we have crystallised various peptides, especially circular ones, both from
regular screens used in MX and from specially prepared screens (i.e. different
organic solvents).
We tend to use slow evaporation from under mineral oil in Terazaki plates (i.e.
microbatch).
We have also
NMR ... synthesize with a few labeled aa according to taste of local
NMR guru.
If they cant do a15-aa peptide in a day, let the bb know, it will be
entertaining ;-)
A.
On May 25, 2011, at 14:04, Buz Barstow wrote:
Dear all,
I am considering trying to crystallize a small peptide (around
I would discourage using pre-made screens on a project outside the norm.
The main problems are:
Zinc: At mM concentrations will easily crystallize and give false positives
in many screens. It will also act as a precipitant for the peptide.
The best approach would be to separate the zinc adduct
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Buz Barstow
Sent: Wednesday, May 25, 2011 8:04 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Peptide Crystallization
Dear all,
I am considering trying to crystallize a small peptide (around 15 amino
acids). The peptide is soluble in neutral water or buffer (pH
