Re: [ccp4bb] Queries regarding bead beater and french press.

2015-01-14 Thread Johnson Luwang
Thanks to all. I noted down all the information you shared with us. Cheers! Johnson L.W On Tue, Jan 13, 2015 at 3:08 PM, Valerie Biou valerie.b...@ibpc.fr wrote: hello we have a French press, a bead machine and constant CellD disruptor in our institute. I can tell you that most people

Re: [ccp4bb] Queries regarding bead beater and french press.

2015-01-13 Thread Valerie Biou
hello we have a French press, a bead machine and constant CellD disruptor in our institute. I can tell you that most people have switched to the Constant system because it works fine and is so much easier and faster to run and clean. people disrupt yeast without any problem. all the best

[ccp4bb] Queries regarding bead beater and french press.

2015-01-12 Thread Johnson Luwang
Dear All, I am looking for an equipment that can do *Saccharomyces cerevisiae* cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment.

Re: [ccp4bb] Queries regarding bead beater and french press.

2015-01-12 Thread Jurgen Bosch
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Re: [ccp4bb] Queries regarding bead beater and french press.

2015-01-12 Thread Roger Rowlett
The Bead Beater has a 15, 40, and 350 mL chambers. I haven't used mine to homogenize yeast, but I suspect it is similar in performance to E. coli disruption. (Different bead sizes are used for yeast than bacteria.) We get excellent, gentle disruption of E. coli in 8 minutes total. A French