Dear All,
Sorry for the non crystallography related question
We are performing a fluorescence based assay to screen for inhibitor
compounds of our enzyme and ultimately crystallize the enzyme along with
inhibitor.
We see that some of our compounds are autofluorescent and thus are effecting
You are measuring fluorescence changes which are likely to be due to
compound binding by your enzyme.
In this case your blank must be your compound blank and no other.
Then you measure changes in fluorescence intensity and lambda max of
emission as you add your enzyme.
I do not know you
