Re: [ccp4bb] Should I be worried about negative electron density?

2010-05-19 Thread Clemens Vonrhein
Hi Jay, there have been a few discussions about sigma/rms levels, absolute values (e/A*3), significance levels etc on ccp4bb. See e.g. http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg06969.html http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg15273.html which are the ones I could

Re: [ccp4bb] Should I be worried about negative electron density?

2010-05-19 Thread Thomas Womack
On 19 May 2010, at 00:36, Paul Emsley wrote: Jay Pan wrote: Hello Everyone, I have a reasonably well fitted electron density map through molecular replacement. However, there is always some red region left no matter how hard I tried when the mtz file is loaded into Coot. Is this because

[ccp4bb] Should I be worried about negative electron density?

2010-05-18 Thread Jay Pan
Hello Everyone, I have a reasonably well fitted electron density map through molecular replacement. However, there is always some red region left no matter how hard I tried when the mtz file is loaded into Coot. Is this because my model is still not good enough or it’s natural to most model

Re: [ccp4bb] Should I be worried about negative electron density?

2010-05-18 Thread William G. Scott
On May 18, 2010, at 4:13 PM, Jay Pan wrote: Hello Everyone, I have a reasonably well fitted electron density map through molecular replacement. However, there is always some red region left no matter how hard I tried when the mtz file is loaded into Coot. Is this because my model is