Just some ideas. NCS usually good with 2.7A data, TLS only to be used
when model is complete..
You say Matthews suggest 11 molecules, but you only find 6 - doesnt
this mean you have a very high solvent content?
I would check the MR carefully.
How are the 6 molecules related - are there
Hello again...
I have a 2.7A resolution data set. Spacegroup P212121 as suggested by Pointless
and best space group when run through Phaser. Unit cell is
110.02x160.49x186.55. Mathew's suggest 11 complexes in ASU. Both Molrep and
Phaser can only find 6. This seems to be a typical observation
I'd use NCS in the beginning and maybe also DM solvent flattening,
then build and fix your structure, run refmac without NCS, superimpose
your chains in Coot and re-define your strict NCS groups accordingly.
Therafter you can submit your current coordinates to Ethan's TLSMD
server and