Hi everyone,
I think my first post did not clearly describe the machine I need. It's
very kind of Zhijie and other repliers to reminde me this problem. So I
post it again here, try to make it short and clear. Sorry for occupying the
space of your mailbox twice.
Actually I need a chamber like a culture incubator (regular one for
E.coli growth), but this chamber must have both cooling and heating
function (b.c. I need low temprature like 4C), and running quietly
(vibration seems not good for my crystal).
The procedue is like: I put my apparatus into the chamber, set the
temperature inside to 4C for 2 hr, and then 25C for 2 hr, and back to 4C
for 2hr, and again 25C for 2hr, and so on... So I also need it can be
programmed to make a temperature cycling.
Thanks for Zhijie again. She/he gave me a lot of help and finally find me
this one http://www.scientificindustries.com/envirogenie.html
I think except it has not programmed temperature cycling control,
every other feature of this machine is perfect, especially it removes
compressor (it uses *Peltier controlled Refrigeration and Heating*). Does
anyone know better choice for me?
Jun Ma
-- Forwarded message --
From: Zhijie Li zhijie...@utoronto.ca
Date: 2012/6/13
Subject: Re: [ccp4bb] Where to get a thermo-cycling controlled chamber
To: Jun Ma mrmar2...@gmail.com
**
Hi Jun,
You had better send this last reply of yours to the CCP4BB. I think this
one describes your requirement more clearly than the original post.
BTW, I think there must be lots of refrigerated incubators with computer
interface around, which you can program the thermo cycles. I found
the following one with a serial port(RS232) for controlling online:
http://www.scientificindustries.com/envirogenie.html
But when there is a built-in compressor, I doubt how quiet it could be.
Another concern is, heat conduction by air is always very slow, unless you
have a fan to blow air over your apparatus, which also brings vibrations to
your system.
Zhijie
*From:* Jun Ma mrmar2...@gmail.com
*Sent:* Wednesday, June 13, 2012 5:59 PM
*To:* Zhijie Li zhijie...@utoronto.ca
*Subject:* Re: [ccp4bb] Where to get a thermo-cycling controlled chamber
Hi Zhijie,
Thank you again for quick response. I don't need to change temprature too
quickly. Actually a chamber like a culture incubator (for E.coli growth) is
good for me, but this chamber must have cooling function (b.c. I need low
temprature like 4C), and running quietly (vibration seems not good for my
crystal).
The procedue is like: I put my apparatus into the chamber, set the
temperature inside to 4C for 2 hr, and then 25C for 1 hr, and back to 4C
for 2hr, and again 25C for 1hr, and so on...
I don't know if I describe what I want clearly this time. I have spent a
lot time for looking for this chamber. I think there must be
someone knowing something, out of so many experienced experts on CCP4.
Thank you again for taking your time and thoughts to help me.
Jun
2012/6/13 Zhijie Li zhijie...@utoronto.ca
**
Hi Jun,
How fast do you need the temperature to be changed? One easy way to get
very fast temperature change is to prepare water at different temperatures,
and circulate the water around your chamber, which of course need to be
watertight. If you can find valves that can take computer instructions then
you can control the sequence of the water of different temperatures going
around your chamber. If you have a constant temperature hot water supply
and ice water supply and are not t.o concerned about wasting water, then
two proportional valves can do what you want: mix the ice water with the
hot water at different ratios then you can get to anywhere in the 0-50C
range.
I doubt there would be solid block based devices that would allow you to
quickly and evenly change your chamber's temperature, as this would need
even and efficient heating and cooling of your chamber on all 6 faces,
which is difficult to do with solid surfaces.
Zhijie
*From:* Jun Ma mrmar2...@gmail.com
*Sent:* Wednesday, June 13, 2012 3:36 PM
*To:* Zhijie Li zhijie...@utoronto.ca
*Subject:* Re: [ccp4bb] Where to get a thermo-cycling controlled chamber
Hi Zhijie,
Thank you for your detailed reply. I'm sorry i didn't describe as clearly
as possible. I'm trying to make 2D-crystal, and using a special apparatus,
not so big, but cannot fit a regular thermocycler designing for
96-wells plate/PCR. So I need a kind of chamber, in which I can put my
apparatus. Do you have any idea about this?
Jun
2012/6/13 Zhijie Li zhijie...@utoronto.ca
**
Hi Jun，
In this following article an metallic adapter was used to mount a 192
well sitting drop plate on a conventional thermocycler.
http://journals.iucr.org/d/issues/2006/05/00/av5047/av5047bdy.html.
However I guess there would be significant evaporation and condensation
if you use vapor diffusion setup and cycle the temperatures.
On the other hand, if you do microbatch under oil, then you
