Hi all
Just wanted to thank you all for all the suggestions and tell you of all
the things i tried what finally worked, if anyones interested.
Among the several things mentioned here, the crystals diffracted the best
(~ 2.9 Å at the synchrotron) when grown in a solution containing either 5%
ethyl
Message-
> From: Mahesh Lingaraju
> To: CCP4BB
> Sent: Wed, Jan 22, 2014 10:38 am
> Subject: [ccp4bb] cryoprotection ideas for salt based condition
>
> Hello folks,
>
> I have crystals for a protein which form in 0.1 M MES pH 6-6.75, 10%
> dioxane and 1.6-2 M Ammonium su
araju
To: CCP4BB
Sent: Wed, Jan 22, 2014 10:38 am
Subject: [ccp4bb] cryoprotection ideas for salt based condition
Hello folks,
I have crystals for a protein which form in 0.1 M MES pH 6-6.75, 10% dioxane
and 1.6-2 M Ammonium sulphate. Based on little experience I have finding the
Mahesh,
Try 25-30% glucose. You can gradually add well solution + 37.5-40%
glucose directly to the drop if your crystals are sensitive to changes
in osmotic pressure. If your drop is evaporating too quickly, try
working in the cold room or under oil to slow down evaporation. You can
find our
Hello folks,
I have crystals for a protein which form in 0.1 M MES pH 6-6.75, 10%
dioxane and 1.6-2 M Ammonium sulphate. Based on little experience I have
finding the right cryoprotection for salt based conditions; I have tried
glycerol (15-25%), Ethylene glycol (20-25%), DMSO (15-20%) and increas
