We had a P1 case with 8 molecules/asu and the crystal diffracted to 2A
resolution. Initial indexing showed that the data could be indexed in P212121
space group but the data could not be merged in this space group (Rsym 60%). P1
was the only space group that we could merge the data (Rsym 10%).
Here is an example that is is not cryocongelation but spacegroup change
upon cooling :
Garavito RM, Jenkins J, Jansonius JN, Karlsson R, Rosenbusch JP. X-ray
diffraction analysis of matrix porin, an integral membrane protein from
Escherichia coli outer membranes. J Mol Biol. 1983 Feb 25;164(2):31
we had a P1 case with 12 mols/au, about 30% sequence id, 2.4A
resolution, which was solved after a lot of trials with phaser. Other
problems of these crystals were that they took months to grow and
invariable presented multiple lattices (we did not try microbeam).
See:
Crystallization of t
Mario,
As others have asked, why/how did you decide on P1? You mentioned
pseudo-translation being present. Depending on the location in the
Patterson map this could be a pseudo-centering operator showing an
apparent I4 space group. If this is the case you may want to reindex in
the P4 cell
CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
elizabeth.d...@diamond.ac.uk
Sent: Thursday, September 30, 2010 9:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] difficult P1 crystal
no publication springs to mind immediately (often apparently of non
scientific relevance) but I hav
There are a couple of papers...
Acta Cryst. (2010). F66, 346-351
Crystallization and X-ray diffraction studies of cellobiose phosphorylase from
Cellulomonas uda
The space group was originally P21. During collection the crystal moved out of
the beam (and possibly the cyrostream). Upon recenter
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
elizabeth.d...@diamond.ac.uk
Sent: Thursday, September 30, 2010 9:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] difficult P1 crystal
no publication springs to mind immediately (often
Liz
From: CCP4 bulletin board on behalf of Sanishvili, Ruslan
Sent: Thu 30/09/2010 15:03
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] difficult P1 crystal
Hi James,
Can you, or anybody else, point me to a publication where "...not that
uncommon for one or mo
gonne, IL 60439
Tel: (630)252-0665
Fax: (630)252-0667
rsanishv...@anl.gov
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
James Holton
Sent: Thursday, September 30, 2010 8:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] difficult P1 crystal
Yes
Yes, this sort of thing happens a lot more often than one might think,
but people who have crystals with such "high-copy asymmetric units" tend
to not solve them. Hence, they don't end up in the PDB. In cases where
the structure is eventually solved, it is usually done by finding an
alternati
Hello Mario,
at 1.9A you might even give S-SAD a try, especially if you have access to an
inhouse source with the flexibility to collect data with a (real) high
multiplicity.
Tim
On Thu, Sep 30, 2010 at 02:40:26PM +0200, Mario Milani wrote:
> Thank you for the suggestions. The data resolution is
What does this mean?
You index what looks to be tetragonal, do your collection, and then it
integrates/scales only in P1?
F
On Sep 30, 2010, at 5:54 AM, Mario Milani wrote:
> It initially looks like tetragonal (I4, a=141, b=141, c=208) and then
> results triclinic (P1, a=141, b=141 c=144, al
Thank you for the suggestions. The data resolution is 1.9 so i can try
different heavy atoms techniques ... anyway i am really puzzled by the
peculiar assembly in the crystal and on the possible causes... does anyone
know about similar cases?
mario
>
> Mario,
> beside what you were mentioning, I w
Dear Mario,
what is the resolution of the data? Could you try SeMet-MAD/SAD or some other
phasing method to overcome the MR-problem?
Tim
On Thu, Sep 30, 2010 at 12:54:36PM +0100, Mario Milani wrote:
> Dear all,
> i have a 30 kDa protein that crystallize so far in three different conditions
> b
Mario,
beside what you were mentioning, I would definitely try a quick soak (10-30
seconds) of the crystals in cryo conditions supplemented with halides such as
NaBr, or NaI, at pretty high concentrations (say 0.5 M), then directly freezing
without backsoak.
If the crystals survive the treatment
Dear all,
i have a 30 kDa protein that crystallize so far in three different conditions
but with the same space group. It initially looks like tetragonal (I4, a=141,
b=141, c=208) and then results triclinic (P1, a=141, b=141 c=144, alpha=119,
beta=119, gamma=90), hosting about 24 mol. in the uni
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