Thank you to everyone for the helpful suggestions
From: Yingyun Liu
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, February 28, 2013 10:29 PM
Subject: Re: [ccp4bb] disulfide engineering
I have used a copper solution that worked well. The details can be found in
] disulfide engineering
To: CCP4BB@JISCMAIL.AC.UK
> In the literature, you can find examples of air oxidation, oxidized
> glutathione (alone), mixture of reduced and oxidized glutathione, and
> hydrogen peroxide. The correct concentrations have to be found
> empirically. We are just
da Pacific
Subject: Re: [ccp4bb] disulfide engineering
Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the "anti-DTT?"
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs < drdavidcbri...@gmail.com >
wrote:
You might want to try &q
I don't know how much mileage you'd get out of it with your protein, but
I was able to get very efficient disulfide linkage at the dimerization
interface of my protein by dropping the salt to nearly nothing and
running lots of buffer over it after immobilization on a cation exchange
column (dur
Dera Careina
going back to the original software question, I think you may be able to use
the Rasmot-3D Pro server
http://biodev.cea.fr/rasmot3d/
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W459-64. doi:
10.1093/nar/gkp304. Epub 2009 May 5.
RASMOT-3D PRO: a 3D motif search webserver.
Debr
In the literature, you can find examples of air oxidation, oxidized
glutathione (alone), mixture of reduced and oxidized glutathione, and
hydrogen peroxide. The correct concentrations have to be found
empirically. We are just now mushing through this with an engineered
disulfide variant. Air ox
l.mce...@evotec.com
> Tel: +44 (0)1235 861561
> Fax:+44 (0)1235 863139
> direct line: +44 (0)1235 838802
> www.evotec.com
>
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
> Keller
> Sent: 28 February 2013 11:09
> To: CCP4BB@JISCMAIL
Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the "anti-DTT?"
Glutathione (red) + Glutathione (ox), redox potential is adjusted by
varying the ratio.
Best,
Clemens
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs
wrote:
You might want to try "Dis
CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] disulfide engineering
Along these lines, what reagents do people use to promote disuflide
bonds, i.e., the "anti-DTT?"
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs
wrote:
You might want to try "Disulfide by design"
http:
Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the "anti-DTT?"
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs wrote:
> You might want to try "Disulfide by design"
>
> http://cptweb.cpt.wayne.edu/DbD2/
>
> Cheers
>
> Dave
> On Feb 28, 2013 6:55 AM, "Careina Ed
You might want to try "Disulfide by design"
http://cptweb.cpt.wayne.edu/DbD2/
Cheers
Dave
On Feb 28, 2013 6:55 AM, "Careina Edgooms" wrote:
> Dear CCP4 members
>
> I wish to engineer a disulfide bond at the dimer interface of a protein I
> am working with. Does anyone know of any available sof
Dear CCP4 members
I wish to engineer a disulfide bond at the dimer interface of a protein I am
working with. Does anyone know of any available software to assist with this?
Best
Careina
12 matches
Mail list logo
