Dear Yan:
I had a similar problem with the crystal(thin, thread diffraction in one
direction ). Many try with the crystal optimization failed. However using
micro-focus beamline with a small oscillation angel (0.1- 0.2 degree) I could
collect a full data set and successfully processed with XDS.
CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ??
Sent: Tuesday, May 28, 2013 9:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to make the crystal thicker
Dear professors,
I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350,
however, it is very thin. From
I think there are some mitegen loops that are flat. They will probably be
useful for your crystals given what you say about the 2.2 Å anisotropic
diffraction, which is probably caused by deformation of your plates in
"conventional" loops.
Some others on the list may have a more specific recomme
Instead of making the crystal thicker, why don't you make the beamsize smaller
? Aka microfocus
If this is your cryo-condition then I would work on it a bit more or change the
drop ratio of protein:reservoir so that you can use a higher PEG3350
concentration in your reservoir. I would aim for >2
Dear professors,
I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350,
however, it is very thin. From one side, the diffraction is perfect, about
2.2A, but from the other side, diffraction is too bad, the spots look like a
thread! Process cannot be done by HKL2000.
As t
