Thank you all for your suggestions, I copied them below
I also found a paper for making Src and Abl catalytic domains Protein
Science Dec 2005
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Before, I found a paper of Bhandari R (2000) where they describe the
co-expression of tyrosine kinase EphB1 (attached file). I haven’t checked
how generic the kinase is.
Kind regards
Seppe Leysen
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Guillermo Montoya gmont...@cnio.es
I do not have the full reference here
But there is a febs lett paper from crhistoph muller group where they co
express stat transcription factor with a kinase to obtain the dimer
Dimerisation depends on tyr phosphorylation
Hope it helps
G.
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Hi,
This is in response to your post on the CCP4 bulletin board.
We either express Src independently and mix it with the target protein to
be phosphorylated in the presence of ATP at 37°C (works well) or co-express
the target protein with a non-specific PTK.
Best
Nicolas Nassar, PhD
On Tue, Dec 17, 2013 at 2:13 AM, Edward A. Berry ber...@upstate.edu wrote:
The kinase domain of FAK can be made constitutively active by a mutation
and expressed in E. coli. It's called Super FAK Kinase. But i am afraid
it is rather specific. If you don't get a better lead I can send you the
sequence and a reference.
Gloria Borgstahl wrote:
Does anyone know of a good way to phosphorylate the Tyr on a protein for
structural
studies? Is there a generic kinase that can be coexpressed or purified
for phosphorylation?
The pCMF Amber codon system is very expensive
and Glu really doesn't mimic pTyr all that well.
Any ideas/help would be appreciated, G
