y.hell...@uit.no>
Phone: +47 77646474
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dean
Derbyshire
Sent: 30. april 2014 12:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] metals disapear
Hi all,
Has anyone experienced catalytic metal ions disappearing during data collect
Dear Dean,
I appreciate you might not be able to reveal further details but 'disappearing
>>during<< data collection' sounds interesting as does 'metals' plural (are
they expected to be close together?).
Best wishes,
John
Prof John R Helliwell DSc
On 30 Apr 2014, at 11:33, Dean Derbyshire
emont, IL 60439
>
> Tel: (630)252-0665
> Fax: (630)252-0667
> rsanishv...@anl.gov
>
>
> ________
> From: zbys...@work.swmed.edu [zbys...@work.swmed.edu]
> Sent: Wednesday, April 30, 2014 10:33 AM
> To: Sanishvili, Ruslan
> Cc: ccp4bb@jiscmail.a
Dear All,
So there has been quite a bit of advice on minimising radiation
damage, and on some of the effects of radiation damage, but unless I have
missed it no-one has come up with a clear cut case where radiation damage
actually resulted in the (complete ?) loss of a metal ion.
M/CA@APS
> X-ray Science Division, ANL
> 9700 S. Cass Ave.
> Lemont, IL 60439
>
> Tel: (630)252-0665
> Fax: (630)252-0667
> rsanishv...@anl.gov
>
>
> ____
> From: zbys...@work.swmed.edu [zbys...@work.swmed.edu]
> Sent: Wednesd
swmed.edu]
Sent: Wednesday, April 30, 2014 10:33 AM
To: Sanishvili, Ruslan
Cc: ccp4bb@jiscmail.ac.uk
Subject: Re: [ccp4bb] metals disapear
If metal ion will be sensitive to radiation depends on its redox chemistry
and not its X-ray properties. For a metal to be affected by radiation dose
it needs to
Dear Dean
An example, albeit not a metal, can be found here:-
http://journals.iucr.org/s/issues/2007/01/00/xh5011/xh5011.pdf
Such specific damage has a long history:-
http://www.sciencedirect.com/science/article/pii/0022024888903223
An X-ray sensitive metals centre is the Mn5Ca OEC of PS II and d
___
> From: CCP4 bulletin board [CCP4BB@jiscmail.ac.uk] on behalf of Dean
> Derbyshire [dean.derbysh...@medivir.com]
> Sent: Wednesday, April 30, 2014 5:33 AM
> To: CCP4BB@jiscmail.ac.uk
> Subject: [ccp4bb] metals disapear
>
> Hi all,
> Has anyo
CCP4BB@jiscmail.ac.uk] on behalf of Dean Derbyshire
[dean.derbysh...@medivir.com]
Sent: Wednesday, April 30, 2014 5:33 AM
To: CCP4BB@jiscmail.ac.uk
Subject: [ccp4bb] metals disapear
Hi all,
Has anyone experienced catalytic metal ions disappearing during data collection
?
If so, is there a way
My comments:
Such observation is very uncommon for metals involved in catalysis by
proteins. I have seen quite a few such structures involving Mg, Ca, Fe,
Mn, Zn and most of the radiation damage was not at the catalytic metal. In
case of Fe once I noticed slight shift in the position of the Fe ion
Dear Dean (and Andrew),
I am certainly no radiation damage guru, but at the RD8 Workshop in
Hamburg 3 weeks ago (http://www.rd-eight.org/RD8-01/) I heard a talk by
Pernille Harris about the photoreduction by X-rays of Cu(II) to Cu(I) in a
Cu-insulin crystal.
Tim's description couldn't b
30, 2014 6:33 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] metals disapear
Hi all,
Has anyone experienced catalytic metal ions disappearing during data collection
?
If so, is there a way of preventing it?
D.
Dean Derbyshire
Senior Research Scientist
[cid:image001.jpg@01CF6450.2D6A0D80
Can the radiation damage gurus comment on this ? I know there is a problem with
radiation damage changing the valence state of metals, but I don't remember
hearing about the metal actually being lost due to radiation damage. Is this
really common ?
Thanks,
Andrew
On 30 Apr 2014, at 11:46, Tim
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Dear Dean,
this is probably a very common observation: X-rays produce reducing
electrons and as you reduce a metal I imagine it does not like its
chemical environment as much as it did highly charged.
Everything you can do to avoid radiation damage s
Hi all,
Has anyone experienced catalytic metal ions disappearing during data collection
?
If so, is there a way of preventing it?
D.
Dean Derbyshire
Senior Research Scientist
[cid:image001.jpg@01CF6470.5FA976D0]
Box 1086
SE-141 22 Huddinge
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