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主题： Re: [ccp4bb] methods to capture proteins from cell culture medium
Bei,
I had a former labmate who had the same situation and would load somewhere
between 6-8L of media directly onto a column. I don't remember what type of
column it was, ion exchange may not be ideal
for the project and just used the standard protocol.
Regards,
Mitch
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of joybeiyang
Sent: Tuesday, April 12, 2011 2:14 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] methods to capture proteins from cell culture
Dear all,
My protein of interest was expressed as secreted protein, so I have to collect
the medium and change the buffer with sortorius Jet before I load the sample
onto a IMAC, the buffer change step in my current protocol can last for 12hrs
(I have to concentrate 4L to 200ml, then dilute
column.
Good luck,
Mike
- Original Message -
From: joybeiyang joybeiy...@gmail.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, April 12, 2011 2:13:49 PM GMT -08:00 US/Canada Pacific
Subject: [ccp4bb] methods to capture proteins from cell culture medium
Dear all,
My protein of interest
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主题： Re: [ccp4bb] methods to capture proteins from cell culture medium
Bei,
I had a former labmate who had the same situation and would load somewhere
between 6-8L of media directly onto a column. I don't remember what type of
column it was, ion exchange may not be ideal if the ionic
I thought about HIC too, but do not know if it would work since the
binding of protein to HIC need high salt conc. and I am not sure if the
salt conc. in the sf900 or Hi5 medium is high enough (the formulation is
secret, LOL), thus it is good to know that someone has succesful
experience
