Deal all,
Sorry for the non-ccp4 query once again.
I need suggestions regarding the improvement in crystal quality. I have
crystallized a protein in MPD. The crystals grow like a thin plates and the
plates are stacked together. So, the mosaicity is very high and also
indexing is difficult even at
James,
I think the standard suggestions apply. Try to tweak your
crystallization conditions to get a single crystal (i.e. not a series of
stacked plates). PEG 400 can be a good substitute for MPD, but you
could also try additive screens as well as co-crystallization with
products,
We had a similar situation: great diffraction,
multiple lattices due to stacked plates that appeared to be single
crystals. We ran an additive screen with about a dozen common salts and
solvents, e.g., AmSO4, LiCl, MgSO4, AmPO4, PEG400, EG, glycerol, DMSO,
etc. at 100 mM for salts or 5-10% for
Have you tried using these crystals to produce seeds for matrix seeding?
charlie
Deal all,
Sorry for the non-ccp4 query once again.
I need suggestions regarding the improvement in crystal quality. I have
crystallized a protein in MPD. The crystals grow like a thin plates and
the
plates
