Re: [ccp4bb] structure determination from a hollow crystal

2016-11-30 Thread Chris Fage
Hi Eike, I wouldn't necessarily let the morphology deter you. While working on my PhD, a colleague collected beautiful data from hollow crystals. If the crystals collapse while looping, is it possible for you to perform "micro-surgery" on the crystal so that you only have a single face?

[ccp4bb] structure determination from a hollow crystal

2016-11-29 Thread Schulz, Eike-Christian
Dear all, Is there a documented case of a structure determination from a hollow crystal? We have the unfortunate situation that a protein only crystallizes as a hollow tube, where the inside is filled with solvent, which ruins the diffraction patterns. I was often confronted with similar