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> hi sreetama,
>
if ur protein is making aggregates during
crystallization try to add glycerol, EDTA in Ur buffer. R u purifying ur
protein from Gelfiltration column ?is ur protein is coming in proper place?
if every thing is right then u can try DTT and TCEP in ur buffer
Dear all,
I have a 20 KDa protein that has a single cys residue , about 42
residues from the C-terminal . The protein aggregates during crystallization
setup ( hanging drop vapour diffusion) . What ratio of protein and
mercaptoethanol is to be used to avoid aggregation and help in cr
Dear all,
I have a 20 KDa protein that has a single cysteine , about 42
residues from the C - terminal . The protein aggregates during crystallisation
setup (hanging drop, vapour diffusion ) . What ratio of protein and
mercapto-ethanol should be used during trials to prevent aggregat