Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

] ** ** Headington, Oxford OX3 7BNhttp://www.oppf.ox.ac.uk ** Original message >Date: Tue, 8 Apr 2008 13:42:21 +0200 >From: Kay Diederichs <[EMAIL PROTECTED]> >Subject: Re: [ccp4bb] which concentrated salt has lowest vapour pressure? >To: CCP4BB@JISCMAIL

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

Dear all, once again this bulletin board proved to be a great ressouce. I obtained 15 emails since I posted - thanks to everybody! I composed a summary for the CCP4 wiki, which can be found at:

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

George M. Sheldrick wrote: Li salts tend to increase the solubility of peptides (Seebach et al., Helv. Chim. Acta 72 (1989) 857-867), which is a pity, because they can also be used as cryoprotectants. George But that is not an issue if you add the salt only to the reservoir. The same is not

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

Kay, Go with LiCl as a reservoir solution (not necessarily in the protein drop). It can generate a very low water vapor pressure. It will suck the water out of almost anything. The CRC handbook has all the information although buried deep within it. However, the DNA and polysacchari

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

Li salts tend to increase the solubility of peptides (Seebach et al., Helv. Chim. Acta 72 (1989) 857-867), which is a pity, because they can also be used as cryoprotectants. George Prof. George M. Sheldrick FRS Dept. Structural Chemistry, University of Goettingen, Tammannstr. 4, D37077 Goettin

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

I'm not entirely sure this paper will answer your question, as I can't access the full article at home, but I seem to remember it may contain what you seek. It's certainly worth giving it another airing... http://scripts.iucr.org/cgi-bin/paper?S0907444905002726 Acta Cryst. (2005). D61, 490-493

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

We normally use AmS or glycerol. Poul On 07/04/2008, at 15.46, Kay Diederichs wrote: Dear all, a protein which we work on is available in low quantity. The only crystallization screen we set up is completely clear, no precipitate, nothing. Now we would like to modify the reservoirs of th

[ccp4bb] which concentrated salt has lowest vapour pressure?

Dear all, a protein which we work on is available in low quantity. The only crystallization screen we set up is completely clear, no precipitate, nothing. Now we would like to modify the reservoirs of this screen, by adding LiCl or Ammoniumsulfate or ... , with the goal of reducing the vapou