[ccp4bb] Crystal handling

2012-04-07 Thread Theresa Hsu
Hi all. I have small and fragile membrane protein crystals with size 40 micron. They like to break when I try take them out with regular loops for freezing. Is there any special tricks to handle them for data collection? Thank you. Theresa

Re: [ccp4bb] very informative - Trends in Data Fabrication

2012-04-07 Thread Jrh
Dear Ron, Quite so, and who cannot laugh at the Yes Minister perfect hospital ward operating theatre sketch ( Thankyou James W). Anyway:- Let's not get too hung up on one detail of your point 3. Your various points, including point 3, added several missing elements in this CCp4bb thread.

[ccp4bb] Refmac executables - win vs linux in RHEL VM

2012-04-07 Thread Bernhard Rupp (Hofkristallrat a.D.)
Something the developers might be interested in: The Refmac_5.6.0117 32-bit windows binaries run native on a win64 3-4x slower than those from the linux distribution run **in a RHEL6.2-64 VMware virtual machine hosted the same windows7/64 system.** VM/RHEL: Refmac_5.6.0117: End of

Re: [ccp4bb] Refmac executables - win vs linux in RHEL VM

2012-04-07 Thread Roger Rowlett
I don't know the state of current software, because I haven't tried recently, but when I set up my student crystallography workstations a few years back I noticed many packages (e.g. EPMR, Phaser) that had potentially long run times (where it is really noticeable) would run on the identical

Re: [ccp4bb] Refmac executables - win vs linux in RHEL VM

2012-04-07 Thread Nat Echols
On Sat, Apr 7, 2012 at 9:50 AM, Roger Rowlett rrowl...@colgate.edu wrote: I don't know the state of current software, because I haven't tried recently, but when I set up my student crystallography workstations a few years back I noticed many packages (e.g. EPMR, Phaser) that had potentially

Re: [ccp4bb] very informative - Trends in Data Fabrication

2012-04-07 Thread Eric Bennett
I doubt many people completely fail to archive data but maintaining data archives can be a pain so I'm not sure what the useful age of the average archive is. Do people who archived to tape keep their tapes in a format that can be read by modern tape drives? Do people who archived data to a

Re: [ccp4bb] A little perspective (Re: Trends in Data Fabrication)

2012-04-07 Thread Bosch, Juergen
along those lines one should also look at this NRDD paper: http://www.nature.com/nrd/journal/v11/n3/full/nrd3681.html Rather long but has lots of very useful citations. If you ask industry people it's a know problem that 50% of their own experiments can't be reproduced but it's even worse if you

Re: [ccp4bb] Crystal handling

2012-04-07 Thread Ho Leung Ng
Have you tried the MiTeGen Micromesh? They're my favorite for handling tiny crystals. Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry h...@hawaii.edu On Sat, Apr 7, 2012 at 1:00 PM, CCP4BB automatic digest system lists...@jiscmail.ac.uk wrote: Hi

Re: [ccp4bb] Crystal handling (Summary)

2012-04-07 Thread Theresa Hsu
Thank you for all the on and off-board replies. The suggestion is to use meshed loops from Molecular Dimensions or Mitegen. I presume both works well to collect data. Theresa