Dear all,
I am shocked by my own ignorance, and you feel free to do the same, but
do you agree with me that according to Bragg's Law
a diffraction maximum at an angle theta has contributions
to its intensity from planes at a spacing d for order 1,
planes of spacing 2*d for order n=2, etc. etc.?
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Dear Pietro,
You may take a textbook into account which deals with Laue
diffraction. If you search for the keyword Laue and the author
Helliwell at the IUCR journals, you will get a large number of hits,
indicating that this is by no means a
Dear Pietro,
Ladd Palmer book does explain it, just first example that springs to mind.
HTH
D
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pietro
Roversi
Sent: 20 August 2013 15:37
To: ccp4bb
Subject: [ccp4bb] Dependency of theta on n/d in
Postdoc in Structural Biology of Membrane Proteins
A postdoc position is available immediately at the Skirball Institute, New York
University School of Medicine, to study the mechanism of membrane transporters
using X-ray crystallography in combination with various biochemical and
biophysical
Hi Evgeny
I do have a few free cysteine residues but i am not sure if this is the
problem in my case, I say that because i have crystallized this protein
with another ligand without any issues and if oxidation of cysteine
residues is a problem, i would imagine that it would have happened in the
Wow! Unexpected advices! Thank you very much, for addon and Fab!
Ed, you are right in both cases: about my name and protease. I think it is
good idea to try to screen for crystallization conditions with bME.
Mahesh, I know nothing about your object, as well as buffer conditions. May
be you would
Hi All,
I have three datasets of SeMet-incorporated protein at peak, infl and high
wavelength respectively. SAD with peak dataset works well to solve the
phase problem. However, MAD with all three datasets didn't work at all. The
completeness of all three datasets are more than 99%. So I think
Dear Yafang,
If radiation damage is not a major problem, MAD should give you more
phase information than SAD, i.e. better maps, especially
at low resolution. If SAD works but MAD doesn't, there are several
possible explanations:
1. (most likely) your datasets are inconsistently indexed. This
Dear George,
if #4 is correct, shouldn't he be able to get good SIRAS using the peak dataset
as HA and the last collected dataset as native
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns
