Hi All, I have three datasets of SeMet-incorporated protein at peak, infl and high wavelength respectively. SAD with peak dataset works well to solve the phase problem. However, MAD with all three datasets didn't work at all. The completeness of all three datasets are more than 99%. So I think radiation damage should not be a problem. Does anyone have any idea about the possible reasons that MAD didn't work in this case? Thank you so much for any of your help!
Best, Yafang -- Yafang Chen Graduate Research Assistant Mesecar Lab Department of Biological Sciences Purdue University Hockmeyer Hall of Structural Biology 240 S. Martin Jischke Drive West Lafayette, IN 47907
