Dear All,
Just wondering what the current situation on CrystalClear tray sealing tape is.
Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips
precut for plates - I guess related with the fact that the consumer box sealing
tape changed specifications and now clouds
Hi Mark,
last time I checked (few months back), Hampton still sold tape.
Shipping to Europe is extortionate but if you combine with screens and
other goodies, it's just about bearable.
Andreas
On 01/12/2014 10:24, Mark J van Raaij wrote:
Dear All,
Just wondering what the current
you are right, I must have been looking with my ears when I checked this
morning.
https://hamptonresearch.com/product_detail.aspx?cid=10sid=88pid=271
Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34)
Dear all,
I'd like to dump header information of a diffraction image file.
CCP4 diffdump is a good tool, though it outputs the same angle
for both oscillation start and end for .cbf image. (bug?)
Are there any other tools to get diffraction image header info?
Regards
-
Takaaki Fukami
Dear Takaaki,
dxtbx provides a way to do this (see
http://journals.iucr.org/j/issues/2014/04/00/jo5001/jo5001.pdf). It is
available in ccp4-python (try 'import dxtbx'), however there is no script
currently in CCP4 that uses this to print the header information. One could
write such a script
Dear Takaaki,
The .cbf images I work with (SLS) have their header as plain text in front of
the image. With an editor like vi I can examine the contents. Also with the
more command you can have a look at it. What I find in the header though, is
Start_angle and Angle_increment; no end angle.
Hi Takaaki
The easy way, since the headers of CBF images are plain ASCII, is to check your
image with a text viewer (e.g. more or less on the command-line). This will
also work for ADSC SMV format, Rigaku d*Trek format, Bruker 86 100
formats, but not for Rigaku original R-Axis format, Agilent
One can use the strings command to show the header:
strings myimage.cbf
Thierry
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
herman.schreu...@sanofi.com
Sent: Monday, December 01, 2014 7:40 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW:
Dear CCP4Sorry for the question, does anyone work with the pMAL-p2X vector not
c2X, because I am trying to find this vector for a long time now, and neither
NEB of life technologies provide this vector. Does anyone know from where I
can get it or any company provides it, I would be grateful.
Dear All,
dxtbx.print_header /path/to/image should work
Graemes-MacBook-Pro:~ graeme$ dxtbx.print_header
data/i04-BAG-training/th_8_2_0001.cbf
=== data/i04-BAG-training/th_8_2_0001.cbf ===
Using header reader: FormatCBFMiniPilatusDLS6MSN100
Beam:
wavelength: 0.97625
sample to source
Hi Mark,
I recently ordered HD Clear Sealing Tape, 3 inches wide from Jena:
http://www.jenabioscience.com/cms/en/1/catalog/287_sealing_tape.html
Best,
Chris
On Mon, Dec 1, 2014 at 12:18 PM, Mark J van Raaij mjvanra...@cnb.csic.es
wrote:
you are right, I must have been looking with my ears
… and there is RigakuReagents, formerly EmeraldBio, too:
https://www.rigakureagents.com/p-595-crystallization-plates.aspx
https://www.rigakureagents.com/p-595-crystallization-plates.aspx
Or google for HD clear duck tape. Several office supply retailers in the US
sell it.
Cheers,
Jan
--
Jan
Hi,
Has anyone used citrate as the sole cryoprotectant? If so,
what concentration was needed?
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
www.khayatlab.org
Dear Rana,
just to point your attention and that of the fellow BB members to a
collection of plasmids (the pCri System) we have prepared and compiled.
They should be available very soon to everybody via Addgene.
Best,
Xavier
Hi Reza,
1.0 and 1.25 M citrate are potential cryo solutions. They still had faint ice
rings present but were not dominant. It is also difficult to make a solution
more concentrated then 1.75 M citrate. Hope this helps.
-Adam
On Dec 1, 2014, at 10:59 AM, Reza Khayat
Hi all,
This plate used to be the Q plate (HR3-124) from hampton research, but I
cannot find them there anymore. Does anyone know where these plates can be
bought from?
Schematic of the plate and its wells is here :
http://www.google.com/patents/US5419278.
Thanks,
F
Hi Reza,
Check the following reference:
Cryoprotection properties of salts of organic acids: a case study for a
tetragonal crystal of HEW lysozyme.
Bujacz G, Wrzesniewska B, Bujacz A.
Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):789-96.
Cheers,
Karolina
W dniu 2014-12-01
Dear crystallographers
Is there any reason for using one data type over the other? Are there any
errors associated with the French and Wilson I-to-F conversion step?
Thanks.
Mohamed
I use 3 clear duck tape. Amazon has it in packages of 6 rolls. Seems to
work OK, with maybe one well in a 2x96 well screen interacting with the
adhesive. I think it's an isopropanol or dioxane condition.
Roger Rowlett
On Dec 1, 2014 5:32 AM, Mark J van Raaij mjvanra...@cnb.csic.es wrote:
Dear
Sent from my iPhone
On Dec 1, 2014, at 14:22, Roger Rowlett rrowl...@colgate.edu wrote:
I use 3 clear duck tape. Amazon has it in packages of 6 rolls. Seems to work
OK, with maybe one well in a 2x96 well screen interacting with the adhesive.
I think it's an isopropanol or dioxane
Institution: Western University of Health Sciences - Pomona Campus
Location: Pomona, CA
Start Date: immediately
Contact: Manal A. Swairjo, Ph.D. mswai...@westernu.edu
We are looking for an outstanding and highly motivated individual to start
immediately in a NIH-funded postdoctoral
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