Dear colleagues,
My lab is hiring a highly motivated PhD student interested in biochemistry and
structural biology of DNA replication proteins. The lab is located in the
Leicester Institute of Structural and Chemical Biology (LISCB), which hosts a
new Cryo-EM facility featuring a 300keV Titan
Dear all,
Due to the recent bad weather we have extended the registration period for
the Spring Symposium: it will now close this Wednesday (7th March). Please
do register by then if you would to attend:
http://www.cvent.com/d/9tq4ln
This conference aims to provide a forum to highlight state of
Dear all,
I am actually dealing with a structure containing an unnatural ligand.
I generated the pdb file by drawing it on PRODRG server, I did a manual
pre-fit in the map using coot and I manually merged the pdf file of my
protein with the one of the ligand.
After that I did few cycles of refinem
Hi Michel,
If your ligand is designated as DRG in your pdb then refinement programs will
anticipate that it is:
Chemical Description
Name5,6-DIHYDRO-BENZO[H]CINNOLIN-3-YLAMINE
Formula C12 H11 N3
Formal charge 0
Molecular weight197.236 g/mol
Component type NON-POLYMER
If this is
Good morning all,
I need to identify my dimer and tetramer interfaces, and point them out in
Pymol. Any website/program/script suggestions? I tried PISA, but it only shows
me some residues from some interfaces, I need something more "complete".
Thank you very much,
Lorenzo
Can you define "more complete"? What information do you require that PISA
does not provide?
This way you may get specific recommendations/suggestions.
Eugene
On 5 March 2018 at 14:02, Lorenzo Briganti
wrote:
> Good morning all,
>
>
> I need to identify my dimer and tetramer interfaces, and po
Hi Colin and Michel,
In my experience, both refmac and coot will use the most recently read-in cif
dictionary and there is no need to try to find an unique identifier for each
new ligand one uses. The new dictionary overrides the old one. Finding a unique
identifier for each new ligand would be
PS: If you have two different home-brewn ligands, you have to rename one of
them (pdb and cif), otherwise the same dictionary will be applied to two
different ligands. Also make sure your cif file is a dictionary and not just a
coordinate file.
HS
Von: Schreuder, Herman /DE
Gesendet: Montag, 5.
Hello all
Does anyone knows of a review that highlights the first examples of the
use of NMR combined with X-ray crystallography to solve the structures
of proteins
thanks
chandra
All,
I was asked by the meeting organizers to let the CCP4 community
know about the 2018 Residential School on Medicinal Chemistry and Biology in
Drug Discovery.See below for information about the course and contact
information.
Sincerely,
Corey
-
Dear all,
Th
Herman is right - as long as refmac reads your generated DRG that is the
dictionary it will use..
My way of doing this sort of thing:
Start with COOT with the DRG coordinates and ProDRG dictionary and try a
bit of real space refinement.
Coot should move the coodinates a bit and you should see som
Llok at this paper:
Does NMR Mean “Not for Molecular Replacement”? Using NMR-Based Search
Models to Solve Protein Crystal Structures
The references there clainm to give the first instance of success...
And Hi Eleanor
On 5 March 2018 at 13:51, Chandra wrote:
> Hello all
>
> Does anyone know
Postdoctoral Position in Biochemistry and X-ray Crystallography
At Rice University
A postdoctoral position in structural biology is available in the Shamoo Lab
at Rice University. The successful candidate must have experience in X-ray
crystallography as well as biochemistry. Candidates should ho
Could it be a member of a coiled coil at some point in its lifetime, as a
part of its function in regulating position or activity of this receptor?
Does the helix have sequence similarity to other coiled coils? see
https://www.uniprot.org/help/coiled for a primer on the topic.
Looks fun!
Emily.
POSTDOCTORAL FELLOW POSITION AVAILABLE
at the Laboratory of Protein Structure of
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY in Warsaw, Poland.
Laboratory of Protein Structure seeks a researcher with a PhD degree to study
the mechanisms of DNA repair.
Laboratory Interest
Laboratory of
PhD STUDENT POSITION AVAILABLE
at the Laboratory of Protein Structure of
INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY in Warsaw, Poland.
Laboratory of Protein Structure seeks PhD student to study the mechanisms of
DNA repair. PhD fellowship is funded in frame of National Science Cent
In addition to the other useful advice offered, I’d also suggest determining if
the whole complex or just the DNA fits in the asymmetric unit. Particularly if
the crystals are hexagonal rods or plates, you might have DNA-only crystals.
~~~
Phoebe A. Rice
Dept. of Bio
Hi All,
We are seeking two junior postdoctoral researchers with demonstrated
expertise in biochemistry and structural biology and an interest in the
structure and function of ligand:receptor and antibody:receptor complexes
in immunity. The appointment will be based at the Monash University
Biomedi
Dear All,
We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which
was co-purified along with our protein.
The expression was done in E.coli.
Any suggestions on how to do this.
Thank you.
Jobi
Hi Jobi,
Phenol/CHCl3 extraction, iPrOH precipitation and then nucleic acid sequencing.
Best regards,Philippe Philippe BENAS, Ph.D.
Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS
Faculté de Pharmacie, Université Paris Descartes
Case 48
Av, de l'Observatoire
F-75270 PARIS cedex
Hi,
I was going to suggest the same but since it has already been said, here’s a
cheeky suggestion: you could try determining the crystal structure of the
complex and get a direct sequence readout for the nuclei acid.
Best,
Debanu
> On Mar 5, 2018, at 9:34 PM, Philippe BENAS
> <0d88e888355
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