[ccp4bb] post doc position at NCMM in Olso, Structural Biology of membrane proteins

2010-06-30 Thread J. Preben Morth
Biology Laboratory (www.embl.org). NCMM is located in brand new laboratories in the University of Oslo and National Hospital Biomedical Campus in the Oslo Research Park and Preclinical Medicine buildings. A new Structural Biology laboratory at NCMM, headed by Dr. J. Preben Morth has been

Re: [ccp4bb] Phenix Autobuild

2010-08-03 Thread J. Preben Morth
use fasta my_amazing_protein THISMESSAGESHOULDHAVEBEENSENTTOTHEPHENIXBB ;-) Preben On 03/08/2010, at 14.19, Md. Munan Shaik wrote: Hi everybody, I have a problem with Phenix autobuild. I want to input sequence file together with the pdb and mtz that I got from molecular replacement. But I

Re: [ccp4bb] R factor R free struck

2010-10-03 Thread J. Preben Morth
hi remember to reindex your data to P21212 in case you used Phaser to search all alternative orthorhombic SG's and it found P22121 Preben On 03/10/2010, at 04.56, Jack Russel wrote: Hi all, I have collected a data at 2.9 Å and the solved the structure using phaser . the space group

Re: [ccp4bb] R factor R free struck

2010-10-04 Thread J. Preben Morth
the reindexing have no effect on the R-factor, I did indeed mean to remember to transform the co-ordiantes according to the indexing :-) On 04/10/2010, at 11.27, Ian Tickle wrote: On Sun, Oct 3, 2010 at 6:34 PM, J. Preben Morth pr...@bioxray.au.dk wrote: hi remember to reindex your data

Re: [ccp4bb] Radiation damage with crystals containing metal centers (TaBr people chime in?)

2010-10-05 Thread J. Preben Morth
? Jacob Keller J. Preben Morth, Ph.D Group Leader Membrane Transport Group Nordic EMBL Partnership Centre for Molecular Medicine Norway (NCMM) University of Oslo P.O.Box 1137 Blindern 0318 Oslo, Norway Email: j.p.mo...@ncmm.uio.no, j...@mb.au.dk Tel: +47 2284 0600 http://www.ncmm.uio.no/research/ncmm

Re: [ccp4bb] phasing with se-met at low resolution

2009-05-11 Thread J. Preben Morth
Dear Engin I would also like to comment. I our recent structure determination of the sodium pump (3.5 A) (see morth JP et al 2007) we did not have experimental phasing to more than 6 A for the Ta6Br12 clusters and 7 A for the Pt sites. Both with extensive multicrystal averaging and phase

Re: [ccp4bb] Phasing at Low Resolution

2009-05-14 Thread J. Preben Morth
Dear Martin You can either add the cluster as a powder, just dip the tip of a needle in the powder and gently approach the drop with the needle, the Ta6Br12 crystals will spray itself onto the drop. Then you just wait for the crystal to turn green, I usually marked the position where the