Hi Jacob,
try adding a bit of edta (1mM) to remove any Ni that might come off the
column. You could also add some DTT or bME to keep cysteines reduced
(careful, add only after the edta!). In my experience the gradient did
not work very weel because the protein with have a lot of impurities.
Dear Xie,
you should seriously consider what Johan Turkenburg suggested:
create a 20~30Gb ext3 patition that you mount as / and the rest of the
disk as ext3 mounted as /home. That way, if you have to reinstall
linux, all your files will be safe. You would end up with the
following
why I should not just merge all these together and
feed them to scala in order to increase multiplicity?
Am I missing something?
Thanks for any comments!
Jose
José Trincão, PhD CQFB@FCT-UNL
2829-516 Caparica, Portugal
It's very hard to make predictions... especially about the future
and multiplicity.
Now, is there any reason why I should not just merge all these together and
feed them to scala in order to increase multiplicity?
Am I missing something?
Thanks for any comments!
Jose
José Trincão, PhD CQFB@FCT-UNL
2829-516 Caparica, Portugal
It's very hard
instead of scaling and
converting with XSCALE and XDSCONV?
Thanks!
Jose
José Trincão, PhD CQFB@FCT-UNL
2829-516 Caparica, Portugal
It's very hard to make predictions... especially about the future - Niels Bohr
Begin forwarded message:
From: Graeme Winter graeme.win...@googlemail.com
Date: March 4, 2011 4/3/11 - 4:51
To: José Trincão trin...@dq.fct.unl.pt
Subject: Re: [ccp4bb] XSCALE vs scala
Hi Jose,
if you are following the usual XDS procedure, the data will have already been
scaled by the XDS
better crystals, is there anything we could try to improve
our data, lower our Rfree?
Thanks!
Jose Trincao
José Trincão, PhD c...@fct-unl
2829-516 Caparica, Portugal
It's very hard to make predictions... especially about the future - Niels Bohr
