Re: [ccp4bb] how to change a membrane protein into a water soluble protein?

Another reference: N. Sukumar, Y.Xu, D.L. Gatti, B.Mitra and F.S. Mathews Structure of an Active Soluble Mutant of the Membrane-Associated (S)-Mandelate Dehydrogenase Biochemistry 40,9870-9878 (2001). In this paper, the membrane protein is converted into the soluble protein by replacing an

Re: [ccp4bb] relationship between B factors and Koff

Sebastiano, We observed similar phenomenon in our protein-protein complex. This reference will give you details (Crystal Structure of an Electron Transfer Complex between Aromatic Amine Dehydrogenase- Azurin from Alcaligenes faecalis, Biochemistry, 45, 13500-13510, 2006). Sukumar Narayanasami