Re: [ccp4bb] disulfide engineering

2013-02-28 Thread Yingyun Liu
I have used a copper solution that worked well. The details can be found in this paper: Nucleic Acids Res. 2004 Sep 30;32(17):5192-7. PMID: 15459288 PMCID: PMC521666 Yingyun Liu - Original Message - From: Roger Rowlett rrowl...@colgate.edu Date: Thursday, February 28, 2013 9:52 am

Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale

2009-09-03 Thread Yingyun Liu
An anion exchange column with a large enough bed volume might be used to concentrate the protein. You probably need to change the pH of the solution so that the protein sticks to the column. On Thu, 2009-09-03 at 17:05 +0800, Xuan Yang wrote: Dear Dr Ku, It was a wonderful idea! However,