An anion exchange column with a large enough bed volume might be used to concentrate the protein. You probably need to change the pH of the solution so that the protein sticks to the column.
On Thu, 2009-09-03 at 17:05 +0800, Xuan Yang wrote: > Dear Dr Ku, > > It was a wonderful idea! However, the refolding buffer contained 500mM > L-Arg and 1mM EDTA. > > And I want to try other affinity columns, just don't know what resin > would be appropriate. > > Sincerely, > > Xuan Yang > > > 2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de> > Can you put a 6His-tag on your protein and add some Ni-NTA > beads to your solution to capture (and concentrate) the > properly folded molecules? > > > > Quoting "Xuan Yang" <pattisy...@gmail.com>: > > Dear All, > > I am working on protein refolding via dialysis in > large volumn > (typically 2~4 litters). It was problematic when I > wanted to concentrate the > solution to at least less than 500ml. If you know any > device appropriate for > such task, please help me out:) > > Thanks in advance! > > Sincerely, > > Xuan Yang > > > >
